2017-Present Publications

Highlights
* = co-first author, † = corresponding author
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NLRP3 cages revealed by full-length mouse NLRP3 structure control pathway activation

Andreeva L*, David L, Rawson S, Shen C, Pasricha T, Pelegrin P, Wu H.†. Cell  184, 1–16 (2021). PDF

"The NACHT-, leucine-rich-repeat- (LRR), and pyrin domain-containing protein 3 (NLRP3) is emerging to be a critical intracellular inflammasome sensor of membrane integrity and a highly important clinical target against chronic inflammation. Here, we report that an endogenous, stimulus-responsive form of full-length mouse NLRP3 is a 12- to 16-mer double-ring cage held together by LRR-LRR interactions with the pyrin domains shielded within the assembly to avoid premature activation. Read More

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Phase separation drives RNA virus-induced activation of the NLRP6 inflammasome

NLRP6 is important in host defense by inducing functional outcomes including inflammasome activation and interferon production. Here, we show that NLRP6 undergoes liquid-liquid phase separation (LLPS) upon interaction with double-stranded RNA (dsRNA) in vitro and in cells, and an intrinsically disordered poly-lysine sequence (K350-354) of NLRP6 is important for multivalent interactions, phase separation, Read More

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Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome formation by sequestering its active C-terminal fragment
 

Sharif H*, Hollingsworth LR*, Griswold AR*, Hsiao JC, Wang Q, Bachovchin DA†., Wu H† Immunity 54: 1-13 (2021). PDF

Inflammasomes are multiprotein complexes that execute pyroptosis in response to cytosolic danger signals. DPP9 regulates the CARD8 inflammasome by unknown mechanisms. Here, Sharif et al. report cryo-EM structures of CARD8 bound to DPP9 and show that this complex inhibits CARD8 inflammasome activation downstream of the proteasome. Read More

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Gasdermin D pore structure reveals preferential release of mature interleukin-1

Xia S, Zhang Z, Magupalli VG, Pablo JL, Dong Y, Vora SM, Wang L, Fu T-M, Jacobson MP, Greka A, Lieberman J, Ruan J,, Wu H†. Nature 593: 607-611 (2021) PDF

As organelles of the innate immune system, inflammasomes activate caspase-1 and other inflammatory caspases that cleave gasdermin D (GSDMD). Caspase-1 also cleaves inactive precursors of the interleukin (IL)-1 family to generate mature cytokines such as IL-1β and IL-18. Cleaved GSDMD forms transmembrane pores to enable the release of IL-1 and to drive cell lysis through pyroptosis. Here we report cryo-electron microscopy structures of the pore and the prepore of GSDMD. Read More

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DPP9 sequesters the C terminus of NLRP1 to repress inflammasome activation

Hollingsworth, L.R.*, Sharif, H.*, Griswold, A.R.*, Fontana P., Mintseris J., Dagbay K.B., Paulo J.A., Gygi S.P., Bachovchin D.A., Wu H.†  Nature 592, 778–783 (2021).  PDF

Nucleotide-binding domain and leucine-rich repeat pyrin-domain containing protein 1 (NLRP1) is an inflammasome sensor that mediates the activation of caspase-1 to induce cytokine maturation and pyroptosis. Gain-of-function mutations of NLRP1 cause severe inflammatory diseases of the skin. NLRP1 contains a function-to-find domain that auto-proteolyses into noncovalently associated subdomains, and proteasomal degradation of the repressive N-terminal fragment of NLRP1 releases its inflammatory C-terminal fragment (NLRP1 CT). Read More

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Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 inflammasomes

Hollingsworth LR*., David L*., Li  Y*., Griswold AR., Ruan J., Sharif H., Fontana P., Orth-He EL., Fu, T., Bachovchin DA, Wu H.Nat Commun 12, 189 (2021). PDF

NLRP1 and CARD8 are related cytosolic sensors that upon activation form supramolecular signalling complexes known as canonical inflammasomes, resulting in caspase-1 activation, cytokine maturation and/or pyroptotic cell death. NLRP1 and CARD8 use their C-terminal (CT) fragments containing a caspase recruitment domain (CARD) and the UPA (conserved in UNC5, PIDD, and ankyrins) subdomain for.. Read More

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HDAC6 mediates an aggresome-like mechanism for NLRP3 and pyrin inflammasome activation

Venkat Giri Magupalli*†, Roberto Negro*†, Yuzi Tian*, Arthur V. Hauenstein*, Giuseppe Di Caprio, Wesley Skillern, Qiufang Deng, Pontus Orning, Hasan B. Alam, Zoltan Maliga, Humayun Sharif, Jun Jacob Hu, Charles L. Evavold, Jonathan C. Kagan, Florian I. Schmidt, Katherine A. Fitzgerald, Tom Kirchhausen, Yongqing Li†, Hao Wu†. Science (2020). PDF

Inflammasomes are supramolecular complexes that play key roles in immune surveillance. This is accomplished by the activation of inflammatory caspases, which leads to the proteolytic maturation of interleukin 1β (IL-1β) and pyroptosis. Here, we show that nucleotide-binding domain, leucine-rich repeat, . Read More

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FDA-approved disulfiram inhibits pyroptosis by blocking gasdermin D pore formation

Hu JJ*, Liu X*†, Xia S, Zhang Z, Zhang Y, Zhao J, Ruan J, Luo X, Lou X, Bai Y, Wang J, Hollingsworth LR, Magupalli VG, Zhao L, Luo HR, Kim J, Lieberman J†, Wu H†.Nature Immunol (2020). PDF

Cytosolic sensing of pathogens and damage by myeloid and barrier epithelial cells assembles large complexes called inflammasomes, which activate inflammatory caspases to process cytokines (IL-1β) and gasdermin D (GSDMD). Cleaved GSDMD forms membrane pores, Read More 

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Gasdermin D activity in inflammation and host defense

Lieberman J*, Wu H*, Kagan JC*. Sci Immunol (2019). PDF

The mechanisms underlying the release of interleukin-1 (IL-1) family cytokines from phagocytes have been the subject of intense investigations for more than 30 years. The absence of an amino-terminal secretion signal from members of this family suggests a previously unknown mechanism of protein secretion that transfers cytosolic IL-1 directly across the plasma membrane into the extracellular space. The pore-forming protein gasdermin D (GSDMD) has emerged as the conduit for... Read More

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Structural Mechanism for NEK7-licensed activation of NLRP3 inflammasome

Sharif H*, Wang L*, Wang WL*, Magupalli VG, Andreeva L, Qiao Q, Hauenstein AV, Wu Z, Núñez G, Mao Y†, Wu H†. Nature (2019). PDF

The NLRP3 inflammasome can be activated by stimuli that include nigericin, uric acid crystals, amyloid-β fibrils and extracellular ATP. The mitotic kinase NEK7 licenses the assembly and activation of the NLRP3 inflammasome in interphase. Here we report a cryo-electron microscopy structure of inactive human NLRP3 in complex with NEK7, at a resolution of 3.8 Å. Read More

Higher-Order Clustering

Higher-Order Clustering of the Transmembrane Anchor of DR5 Drives Signalling

Pan L*, Fu TM*, Zhao W*, Zhao L*, Chen W, Qiu C, Liu W, Liu Z, Piai A, Fu Q, Chen S†, Wu H†, Chou JJ†. Cell (2019). PDF

Receptor clustering on the cell membrane is critical in the signaling of many immunoreceptors, and this mechanism has previously been attributed to the extracellular and/or the intracellular interactions. Here, we report an unexpected finding that for death receptor 5 (DR5), a receptor in the tumor necrosis factor receptor superfamily, the transmembrane helix (TMH) alone in the receptor directly... Read More 

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Crystal structure of the WD40 domain dimer of LRRK2

Zhang P*, Fan Y*, Ru H, Wang L, Magupalli VG, Taylor SS, Alessi DR, Wu H*†. Proc Natl Acad Sci U S A (2019). PDF

Leucine-rich repeat kinase 2 (LRRK2) is a large multidomain protein with both a Ras of complex (ROC) domain and a kinase domain (KD) and, therefore, exhibits both GTPase and kinase activities. Human genetics studies have linked LRRK2 as a major genetic contributor to familial and sporadic Parkinson’s disease (PD), a neurodegenerative movement disorder that inflicts millions worldwide. Read More

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Molecular mechanism for NLRP6 inflammasome assembly and activation

Shen C*, Lu A, Xie WJ, Ruan J, Negro R, Egelman EH, Fu TM*†, Wu H*†. Proc Natl Acad Sci U S A (2019). PDF

Inflammasomes are large protein complexes that trigger host defense in cells by activating inflammatory caspases for cytokine maturation and pyroptosis. NLRP6 is a sensor protein in the nucleotide-binding domain (NBD) and leucine-rich repeat (LRR)-containing (NLR) inflammasome family that has been shown to play multiple roles in regulating inflammation and host defenses. Despite the significance of the NLRP6 inflammasome, little is known about the molecular mechanism behind... Read More

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Conformational flexibility and inhibitor binding to unphosphorylated interleukin-1-receptor-associated kinase 4 (IRAK4)

Wang L*, Ferrao R*, Li Q, Hatcher JM, Choi HG, Buhrlage SJ, Gray NS, Wu H†. The Journal of Biological Chemistry (2019). PDF

Interleukin-1 receptor–associated kinase 4 (IRAK4) is a key player in innate immune and inflammatory responses, performing a critical role in signal transduction downstream of Toll-like receptors and interleukin-1 (IL-1) receptors. Upon ligand binding and via its N-terminal death domain, IRAK4 is ... Read More

2021

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Cryo-EM structures of ASC and NLRC4 CARD filaments reveal a unified mechanism of nucleation and activation of caspase-1

Li Y*, Fu TM*†, Lu A, Witt K, Ruan J, Shen C, Wu H†. Proc Natl Acad Sci U S A (2018). PDF

Canonical inflammasomes are cytosolic supramolecular complexes that activate caspase-1 upon sensing extrinsic microbial invasions and intrinsic sterile stress signals. During inflammasome assembly, adaptor proteins ASC and NLRC4 recruit caspase-1 through homotypic caspase recruitment domain (CARD) interactions, leading to caspase-1 dimerization and activation. Activated caspase-1 processes...Read More

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Structures and gating mechanism of human TRPM2

Wang L*, Fu TM*†, Zhou Y, Xia S, Greka A, Wu H†. Science (2018). PDF

Transient receptor potential (TRP) melastatin 2 (TRPM2) is a cation channel associated with numerous diseases. It has a C-terminal NUDT9 homology (NUDT9H) domain responsible for binding adenosine diphosphate (ADP)–ribose (ADPR), and both ADPR and calcium (Ca2+) are required for TRPM2 activation. Here we report cryo–electron microscopy structures of human TRPM2 alone, with ADPR, and with ADPR and Ca2+. NUDT9H forms both intra- and intersubunit interactions with the N-terminal TRPM homology... Read More 

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DNA melting initiates the RAG catalytic pathway

Ru H*, Mi W*, Zhang P, Alt FW, Schatz DG, Liao M†, Wu H †. Nature structural & molecular biology (2018). PDF

The mechanism for initiating DNA cleavage by DDE-family enzymes, including the RAG endonuclease, which initiates V(D)J recombination, is not well understood. Here we report six cryo-EM structures of zebrafish RAG in complex with one or two intact recombination signal sequences (RSSs), at up to 3.9-Å resolution. Unexpectedly, these structures reveal DNA melting at the heptamer of the RSSs, thus resulting in a corkscrew-like rotation of coding-flank DNA and the positioning of the scissile phosphate in the active site.  Read More

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The Structure of the Necrosome RIPK1-RIPK3 Core, a Human Hetero-Amyloid Signaling Complex

Mompeán M*, Li W*, Li J*, Laage S, Siemer AB, Bozkurt G, Wu H†, McDermott AE†. Cell (2018). PDF

The RIPK1-RIPK3 necrosome is an amyloid signaling complex that initiates TNF-induced necroptosis, serving in human immune defense, cancer, and neurodegenerative diseases. RIPK1 and RIPK3 associate through their RIP homotypic interaction motifs with consensus sequences IQIG (RIPK1) andVQVG (RIPK3). Using solid-state nuclear magnetic resonance, we determined the high-resolution structure of the RIPK1-RIPK3 core. Read More
 

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Cryo-EM structure of the gasdermin A3 membrane pore

Ruan J, Xia S, Liu X, Lieberman J, Wu H†. Nature (2018). PDF

Gasdermins mediate inflammatory cell death after cleavage by caspases or other, unknown enzymes. The cleaved N-terminal fragments bind to acidic membrane lipids to form pores, but the mechanism of pore formation remains unresolved. Here we present the cryo-electron microscopy structures of the 27-fold and 28-fold single-ring pores formed by the N-terminal fragment of mouse GSDMA3 (GSDMA3-NT) at 3.8 and 4.2Å resolutions, and of a double-ring pore at 4.6Å resolution. In the 27-fold pore, a 108-stranded... Read More

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Assembly mechanism of the CARMA1-BCL10-MALT1-TRAF6 signalosome

David L, Li Y, Ma J, Garner E, Zhang X, Wu H†. Proc Natl Acad Sci U S A (2018). PDF

The CARMA1–BCL10–MALT1 (CBM) signalosome is a central mediator of T cell receptor and B cell receptor-induced NF-κB signaling that regulates multiple lymphocyte functions. While caspaserecruitment domain (CARD) membrane-associated guanylate kinase (MAGUK) protein 1 (CARMA1) nucleates B cell lymphoma 10 (BCL10) filament formation through interactions between CARDs, mucosaassociated lymphoid tissue lymphoma translocation protein 1 (MALT1) is a paracaspase with structural similarity to caspases, Read More

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Crystal structure of human IRAK1

Wang L*, Qiao Q, Ferrao R*, Shen C, Hatcher JM, Buhrlage SJ, Gray NS, Wu H†. Proc Natl Acad Sci U S A (2017). PDF

Interleukin 1 (IL-1) receptor-associated kinases (IRAKs) are serine/threonine kinases that play critical roles in initiating innate immune responses against foreign pathogens and other types of dangers through their role in Toll-like receptor (TLR) and interleukin 1 receptor (IL-1R) mediated signaling pathways. Upon ligand binding, TLRs and IL-1Rs recruit adaptor proteins, such as myeloid differentiation primary response gene 88 (MyD88), to the membrane, which in turn recruit IRAKs via the death domains... Read More
 

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AID Recognizes Structured DNA for Class Switch Recombination

Qiao Q*, Wang L*, Meng FL, Hwang JK, Alt FW, Wu H†. Mol Cell (2017). PDF

Activation-induced cytidine deaminase (AID) initiates both class switch recombination (CSR) and somatic
hypermutation (SHM) in antibody diversification. Mechanisms of AID targeting and catalysis remain elusive despite its critical immunological roles and off-target effects in tumorigenesis. Here, we produced active human AID and revealed its preferred recognition and deamination of structured substrates. G-quadruplex (G4)-containing substrates mimicking the mammalian immunoglobulin switch regions are... Read More

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CIDE domains form functionally important higher-order assemblies for DNA fragmentation 

Choi JY*, Qiao Q*, Hong SH, Kim CM, Jeong JH, Kim YG, Jung YK, Wu H†, Park HH†. Proc Natl Acad Sci U S A (2017). PDF

Cell death-inducing DFF45-like effector (CIDE) domains, initially identified in apoptotic nucleases, form a family with diverse functions ranging from cell death to lipid homeostasis. Here we show that the CIDE domains of Drosophila and human apoptotic nucleases Drep2, Drep4, and DFF40 all form head-to-tail helical filaments. Read More

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Cryo-EM structure of the DNA-PK holoenzyme

Sharif H*, Li Y*, Dong Y, Dong L, Wang WL, Mao Y, Wu H†. Proc Natl Acad Sci U S A (2017). PDF

DNA-dependent protein kinase (DNA-PK) is a large protein complex central to the nonhomologous end joining (NHEJ) DNA-repair pathway. It comprises the DNA-PK catalytic subunit (DNA-PKcs) and the heterodimer of DNA-binding proteins Ku70 and Ku80. Here, we report the cryo-electron microscopy (cryo-EM) structures of human DNA-PKcs at 4.4-Å resolution and the DNA-PK holoenzyme at 5.8-Å resolution. The DNA-PKcs structure contains three distinct segments: the N-terminal region with an arm and a bridge... Read More

Other Publications (2017-Present)
* = co-first author, † = corresponding author

Vora SM, Fontana P, Mao T, Leger V, Zhang Y, Fu TM, Lieberman J, Gehrke L, Shi M, Wang L, Iwasaki A, Wu H. ( 2022 ) Targeting stem-loop 1 of the SARS-CoV-2 5' UTR to suppress viral translation and Nsp1 evasion. Proc Natl Acad Sci USA. 119 (9). Doi: 10.1073/pnas.211719819

Xie WJ, Xia S, Warshel A, Wu H. ( 2022 ) Electrostatic influence on IL-1 transport through the GSDMD poreProc Natl Acad Sci U S A. Feb 8;119(6). doi: 10.1073/pnas.2120287119. 

 

Deng W, Bai Y, Deng F, Pan Y, Mei S, Zheng Z, Min R, Wu Z, Li W, Miao R, Zhang Z, Kupper TS, Lieberman J, Liu X. 2022 ) Streptococcal pyrogenic exotoxin B cleaves GSDMA and triggers pyroptosis.  Nature. Feb 2;. doi: 10.1038/s41586-021-04384-4. 

 

Gao W, Li Y, Liu X, Wang S, Mei P, Chen Z, Liu K, Li S, Xu XW, Gan J, Wu J, Ji C, Ding C, Liu X, Lai Y, He HH, Lieberman J, Wu H, Chen X, Li J. 2022 ) TRIM21 regulates pyroptotic cell death by promoting Gasdermin D oligomerization. Cell Death Differ. Feb;29(2):439-450. 

 

Wang L, Crackower MA, Wu H. 2022 ) Advances toward structure-based drug discovery for inflammasome targets. J Exp Med. Jan 3;219(1). doi: 10.1084/jem.20211147.    

 

Vora SM*, Lieberman J, Wu H† (2021). Inflammasome activation at the crux of severe COVID-19.  Nat Rev Immunol. 21(11): 694-703.

Magupalli VG†, Fontana P, Wu H† (2021). Ragulator-Rag and ROS TORment gasdermin D pore formation. Trends Immunol. 42: 948-950. 

Bittner ZA*, Liu X, Mateo Tortola M, Tapia-Abellán A, Shankar S, Andreeva L, Mangan M, Spalinger M, Kalbacher H, Düwell P, Lovotti M, Bosch K, Dickhöfer S, Marcu A, Stevanović S, Herster F, Cardona Gloria Y, Chang TH, Bork F, Greve CL, Löffler MW, Wolz OO, Schilling NA, Kümmerle-Deschner JB, Wagner S, Delor A, Grimbacher B, Hantschel O, Scharl M, Wu H, Latz E, Weber ANR. (2021) . BTK operates a phospho-tyrosine switch to regulate NLRP3 inflammasome activity.  J Exp Med. 218(11). doi: 10.1084/jem.20201656. 

Fillmore N*, Bell S, Shen C, Nguyen V, La J, Dubreuil M, Strymish J, Brophy M, Mehta G, Wu H, Lieberman J, Do N, Sander C. (2021). Disulfiram use is associated with lower risk of COVID-19: (2021) A retrospective cohort study. PLoS One. 16(10): e0259061.

Tong AB, Burch JD, McKay D, Bustamante C, Crackower MA, Wu H. Could AlphaFold revolutionize chemical therapeutics?Nat Struct Mol Biol. 2021 Oct;28(10):771-772. doi: 10.1038/s41594-021-00670-x. PubMed PMID: 34561631.

Gao W, Li Y, Liu X, Wang S, Mei P, Chen Z, Liu K, Li S, Xu XW, Gan J, Wu J, Ji C, Ding C, Liu X, Lai Y, He HH, Lieberman J, Wu H, Chen X, Li J. TRIM21 regulates pyroptotic cell death by promoting Gasdermin D oligomerization. Cell Death Differ. 2021 Sep 11;. doi: 10.1038/s41418-021-00867-z. [Epub ahead of print] PubMed PMID: 34511601.

Sun Z*, Yu H*, Zhao J*, Tan T, Pan H, Zhu Y, Chen L, Zhang C, Zhang L, Lei A, Xu Y, Bi X, Huang X, Gao B, Wang L, Correia C, Chen M, Sun Q, Feng Y, Shen L, Wu H, Wang J, Shen X, Daley GQ, Li H, Zhang J (2021).  LIN28 coordinately promotes nucleolar/ribosomal functions and represses the 2C-like transcriptional program in pluripotent stem cells. Protein Cell . doi: 10.1007/s13238-021-00864-5.

Liu X*,  Xia S*, Zhang Z*,  Wu H, Lieberman J† (2021). Channeling inflammation: gasdermins in physiology and disease. Nat Rev Drug Discov. 20(5): 384-405.

Shen C*,  Vohra M*, Zhang P, Mao X, Figley MD, Zhu J, Sasaki Y, Wu H†, DiAntonio A†, Milbrandt J†. (2021). Multiple domain interfaces mediate SARM1 autoinhibition. Proc Natl Acad Sci USA. 118(4): e2023151118.

Münzer P, Negro R, Fukui S, di Meglio L, Aymonnier K, Chu L, Cherpokova D, Gutch S, Sorvillo N, Shi L, Magupalli VG, Weber ANR, Scharf RE, Waterman CM, Wu H, Wagner DD. (2021). NLRP3 Inflammasome Assembly in Neutrophils Is Supported by PAD4 and Promotes NETosis Under Sterile Conditions. Front Immunol 12: 683803

Wang L, Sharif H*, Vora SM*, Zheng Y, Wu H (2021). Structures and functions of the inflammasome engine. J Allergy Clin Immunol 147: 2021-2029

Andreeva L, Wu H (2021). STING condensates on ER limit IFN response. Nat Cell Biol. 23: 299-300

Xia S*(2020). Biological mechanisms and therapeutic relevance of the gasdermin family. Mol Aspects Med. doi:10.1016/j.mam.2020.100890.

Hatcher JM, Yang G, Wang L, Ficarro SB, Buhrlage S, Wu H, Marto JA, Treon SP, Gray NS † (2020). Discovery of a Selective Covalent IRAK1 Inhibitor with Antiproliferative Activity in MYD88 Mutated B-Cell Lymphoma.  ACS Med Chem Lett .  doi: 10.1021/acsmedchemlett.0c00378

 

Wang L, Wu D, Robinson CV, Wu H, Fu TM(2020). Structures of a Complete Human V-ATPase Reveal Mechanisms of Its Assembly. Mol Cell . doi: 10.1016/j.molcel.2020.09.029

 

Zhao L, Fu Q, Pan L, Piai A, Chou JJ (2020). The diversity and similarity of transmembrane trimerization of TNF receptors. Front Cell Dev Biol. doi: 10.3389/fcell.2020.569684 

Chen H*, Gu L*, Orellana EA, Wang Y, Guo J, Liu Q, Wang L, Shen Z, Wu H, Gregory RI, Xing Y, Shi Y† (2020). METTL4 is an snRNA m6Am methyltransferase that regulates RNA splicingCell Res doi: 10.1038/s41422-019-0270-4.

Xia S*, Hollingsworth LR 4th*, Wu H†(2019). Mechanism and Regulation of Gasdermin-Mediated Cell Death. Cold Spring Harb Perspect Biol. pii: a036400. doi: 10.1101/cshperspect.a036400.

Wu H*, Arnold E* (2019). Michael G. Rossmann (1930-2019) Nat Struct Mol Biol. (8):660-662.

Wang L, Wu H† (2019). Keeping the Death Protein in Check. Immunity. 51(1):1-2.

Scott DA*† , Hatcher JM, Liu H, Fu M, Du G, Fontán L, Us I, Casalena G, Qiao Q, Wu H, Melnick A, Gray NS (2019). Quinoline and thiazolopyridine allosteric inhibitors of MALT1. Bioorg Med Chem Lett. 29(14):1694-1698.

Arnold E†* , Wu H*, Johnson JE*. (2019). Michael G. Rossmann (1930-2019), pioneer in macromolecular and virus crystallography: scientist, mentor and friend. Acta Crystallogr D Struct Biol. 75(Pt 6):523-527.

Xia S*, Wang L*, Fu TM†, Wu H† (2019). Mechanism of TRPM2 channel gating revealed by cryo-EM. FEBS J. 286(17):3333-3339.

Xia S, Ruan J†, Wu H†(2019). Monitoring gasdermin pore formation in vitro. Methods Enzymol. 625:95-107

Shen C, Sharif H, Xia S, Wu H† (2019). Structural and mechanistic elucidation of inflammasome signaling by cryo-EM. Curr Opin Struct Biol. 58:18-25

Lee YR, Chen M, Lee JD*, Zhang J*, Lin SY*, Fu TM, Chen H, Ishikawa T, Chiang SY, Katon J, Zhang Y, Shulga YV, Bester AC, Fung J, Monteleone E, Wan L, Shen C, Hsu CH, Papa A, Clohessy JG, Teruya-Feldstein J, Jain S, Wu H, Matesic L, Chen RH, Wei W, Pandolfi PP† (2019). Reactivation of PTEN tumor suppressor for cancer treatment through inhibition of a MYC-WWP1 inhibitory pathway. Science. 364(6441).

Wang L*, Ferrao R*, Li Q, Hatcher JM, Choi HG, Buhrlage SJ, Gray NS, Wu H† (2019). Conformational flexibility and inhibitor binding to unphosphorylated interleukin-1 receptor-associated kinase 4 (IRAK4). The Journal of biological chemistry. 294(12):4511-4519.

Choi YJ, Kim S, Choi Y, Nielsen TB, Yan J, Lu A, Ruan J, Lee HR, Wu H, Spellberg B, Jung JU†(2019). SERPINB1-mediated checkpoint of inflammatory caspase activation. Nature immunology. 20(3):276-287.

Mompeán M*, Bozkurt G*, Wu H†.(2019). Mimicry by a viral RHIM. EMBO reports. 20(2).

Ru H, Zhang P, Wu H (2018). Structural gymnastics of RAG-mediated DNA cleavage in V(D)J recombinationCurr Opin Struct Biol. 53:178-186.

Orning P, Weng D1*, Starheim K*, Ratner D*, Best Z, Lee B, Brooks A, Xia S, Wu H, Kelliher MA, Berger SB, Gough PJ, Bertin J, Proulx MM, Goguen JD, Kayagaki N, Fitzgerald KA, Lien E†.(2018). Pathogen blockade of TAK1 triggers caspase-8–dependent cleavage of gasdermin D and cell death Science. 362(6418):1064-1069.

Luong P, Hedl M, Yan J, Zuo T, Fu TM, Jiang X, Thiagarajah JR, Hansen SH, Lesser CF, Wu H, Abraham C†, Lencer WI †(2018) INAVA-ARNO complexes bridge mucosal barrier function with inflammatory signaling. eLife. 2018; 7.

Fontán L*†, Qiao Q, Hatcher JM, Casalena G, Us I, Teater M, Durant M, Du G, Xia M, Bilchuk N, Chennamadhavuni S, Palladino G, Inghirami G, Philippar U, Wu H, Scott DA*, Gray NS, Melnick A*† (2018). Specific covalent inhibition of MALT1 paracaspase suppresses B cell lymphoma growth. The Journal of clinical investigation. 128(10):4397-4412.

Feltham R*, Jamal K*, Tenev T*, Liccardi G, Jaco I, Domingues CM, Morris O, John SW, Annibaldi A, Widya M, Kearney CJ, Clancy D, Elliott PR, Glatter T, Qiao Q, Thompson AJ, Nesvizhskii A, Schmidt A, Komander D, Wu H, Martin S, Meier P† (2018). Mind Bomb Refulates Cell Deth during TNF Signalling by Suppressing RIPK1’s Cytotoxic Potential. Cell Rep. 23(2):470-484.

Annibaldi A †, Wicky John S*, Vanden Berghe T*, Swatek KN*, Ruan J, Liccardi G, Bianchi K, Elliott PR, Choi SM, Van Coillie S, Bertin J, Wu H, Komander D, Vandenabeele P, Silke J, Meier P †(2018). Ubiquitin-Mediated Regulation of RIPK1 Kinase Activity Independent of IKK and MK2. Mol Cell. 69(4):566-580.

Xia S*, Fu TM*, Wu H† (2018). Inflammation NODs to Antagonists of RIP2-XIAP Interaction. Mol Cell. 69(4):535-536.

Fu TM*, Shen C*, Li Q, Zhang P, Wu H† (2018). Mechanism of ubiquitin transfer promoted by TRAF6. Proc Natl Acad Sci U S A. 115(8):1783-1788.

Zhang L, Wu H† (2018). Bad germs are trapped. Cell Res. 28(2):141-142

Evavold CL, Ruan J, Tan Y, Xia S, Wu H, Kagan JC† (2018). The Pore-Forming Protein Gasdermin D Regulates Interleukin-1 Secretion from Living Macrophages. Immunity. 48(1):35-44

Hauenstein AV, Xu G, Kabaleeswaran V, Wu H† (2017) .Evidence for M1-Linked Polyubiquitin-Mediated Conformational Change in NEMO. J Mol Biol429(24):3793-3800

Kleino A, Ramia NF, Bozkurt G, Shen Y, Nailwal H, Huang J, Napetschnig J, Gangloff M, Chan FK*, Wu H*†, Li J†, Silverman N*†.(2017). Peptidoglycan-Sensing Receptors Trigger the Formation of Functional Amyloids of the Adaptor Protein Imd to Initiate Drosophila NF-κB Signalin. Immunity 47(4):635-647

Wang H, Feng Z, Lu A, Jiang Y, Wu H, Xu B† (2017). Instant Hydrogelation Inspired by Inflammasomes. Angew Chem Int Ed Engl.56(26):7579-758

Wang L, Qiao Q, Wu H†(2017). Understanding CARD Tricks in Apoptosomes. Structure. 25(4)575-577

Yi L, Bozkurt G, Li Q, Lo S, Menon AK, Wu H† (2017). Disulfide Bond Formation and N-Glycosylation Modulate Protein-Protein Interactions in GPI-Transamidase (GPIT). Sci Rep. 8:45912

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